| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leptin receptor;LEP-R;HuB219;OB receptor;OB-R;CD295;LEPR;DB, OBR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Leptin Receptor, different from the related mouse sequence by nine amino acids, and from the related rat sequence by eight amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes leptin receptor (gene LEPR) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 132.5 kDa, and the supplier reports an observed band at about 150 kDa.
The leptin receptor (LEPR, OB-R) is a class I cytokine receptor that binds the adipocyte hormone leptin. Its long isoform, highly expressed in the hypothalamus, activates JAK2–STAT3 signaling to suppress appetite and increase energy expenditure. Loss of LEPR causes severe obesity, as in db/db mice, and the receptor is central to obesity and metabolic research.
| Target | Leptin receptor (gene LEPR; UniProt P48357, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 132.5 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HeLa cells, human HepG2 cells, human HEL cells, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human leptin Receptor. It differs from the mouse sequence at nine residues and from the rat sequence at eight residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leptin receptor in the cell membrane, basolateral cell membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhou Xihong et al. (2018) Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice. Frontiers in Endocrinology. 10.3389/fendo.2018.00476
S. Pavithra et al. (2023) Leptin decreases the transcription of BKCa channels and Gs to Gi protein-ratio in late pregnant rat uterus. Gene. 10.1016/j.gene.2023.147831
