| Field | Specification |
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| Alternative Names | Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
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| Target |
Overview
Anti-LETM1 Antibody is an antibody targeting Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog (also reported as Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog).
- Immunogen/epitope region: Intracellular, mitochondrial matrix..
- Homology note: Mouse -13/14 amino acid residues identical; human - 12/14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
LETM1 (leucine zipper-EF hand-containing transmembrane region), a protein with a key role in mitochondrial volume homeostasis through regulation of K+/H+ exchange (KHE), is involved in respiratory chain biogenesis and in the pathogenesis of seizures in the Wolf-Hirschhorn syndrome (WHS). LETM1 has been recently proposed to catalyze mitochondrial H+/Ca2+ exchange, which would imply a role in mitochondrial Ca2+ homeostasis as well.LETM1 contains a hydrophobic N-terminal portion spanning the inner membrane and a large hydrophilic portion including the C-terminus located in the matrix. The primary amino acid sequence of the C-terminal part predicts at least two coiled-coil domains and two Ca2+-binding EF hand-like motifs.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NT183.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NT183; Negative control: BLP-NT183.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
