| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich glioma-inactivated protein 1; Epitempin-1; LGI1; EPT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Lgi1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25L55 is a rabbit monoclonal antibody that recognizes leucine-rich glioma-inactivated protein 1 (gene LGI1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 63.8 kDa, and the supplier reports an observed band at about 64 kDa.
LGI1 (leucine-rich glioma-inactivated 1) is a secreted neuronal protein that binds the ADAM22 and ADAM23 receptors at synapses. Through these complexes it regulates Kv1 potassium channels and AMPA receptor-mediated transmission, helping control neuronal excitability. LGI1 mutations cause autosomal dominant lateral temporal lobe epilepsy, and autoantibodies against LGI1 cause a form of autoimmune encephalitis.
| Target | Leucine-rich glioma-inactivated protein 1 (gene LGI1; UniProt O95970, human) |
|---|---|
| Clone | 25L55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.8 kDa |
| Observed MW | 64 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human lgi1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes leucine-rich glioma-inactivated protein 1 as secreted and also located in the synapse, cytoplasm and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in the occipital cortex and hippocampus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
