| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1; KIAA0978 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) is an antibody for ARG1 detection raised in Mouse (Monoclonal, clone Clone: 2B12, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat,Monkey. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ARG1 (additional sex combs like 1,transcriptional regulator); UniProt: P05089
- Antibody format: Mouse, Monoclonal, clone Clone: 2B12, Mouse IgG2b
- Molecular weight: 35 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) catalog # M01106-4.
Biological background
Biological context: Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors,such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG).Acts as coactivator of RARA and RXRA through association with NCOA1.Acts as corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner;the function seems to involve differential recruitment of methylated histone H3 to respective promoters.Acts as corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity (By similarity).Non-catalytic component of the PR-DUB complex,a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).
Expression and localization notes: cellular localization: Nucleus., tissue context: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes..
Common research applications
- Western blotting (WB): Compare ARG1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ARG1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ARG1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes.
- Research category: G Protein Signaling,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
