| Field | Specification |
|---|---|
| Target | |
| Alternative names | SMAD family member 7; SMAD 7; hSMAD7; Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8; MAD homolog 8; Mothers against DPP homolog 8; SMAD7; MADH7; MADH8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MADH7/SMAD7 recombinant protein (Position: R353-Q388). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes SMAD family member 7 (gene SMAD7) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 46.4 kDa, and the supplier reports an observed band at about 50 kDa.
SMAD7 is an inhibitory SMAD that switches off TGF-β and activin signaling. It binds activated type I receptors and blocks access of SMAD2/3, and it recruits the ubiquitin ligase SMURF2 and a phosphatase complex that lead to receptor inactivation, so it acts as a negative feedback regulator in fibrosis, inflammation and cancer studies.
| Target | SMAD family member 7 (gene SMAD7; UniProt O15105, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.4 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human MADH7/SMAD7 fragment (Arg353–Gln388), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SMAD family member 7 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in the lung and vascular endothelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Xia et al. (2024) SIRT1 activation ameliorates rhesus monkey liver fibrosis by inhibiting the TGF-β/smad signaling pathway. Chemico-Biological Interactions. 10.1016/j.cbi.2024.110979
Yang Fan et al. (2017) Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathway in Vitro and in Vivo. Frontiers in Pharmacology. 10.3389/fphar.2017.00929
Yanchun Wei et al. (2019) Astragaloside IV inhibits cardiac fibrosis via miR-135a-TRPM7-TGF-β/Smads pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2019.112404
Naping Hu et al. (2016) Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition. PLoS One. 10.1371/journal.pone.0153409
Y. Tao et al. (2011) Tranilast Prevents the Progression of Chronic Cyclosporine Nephrotoxicity Through Regulation of Transforming Growth Factor β/Smad Pathways. Transplantation Proceedings. 10.1016/j.transproceed.2011.01.160
