| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Macrophage mannose receptor 1; MMR; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Human mannose receptor; hMR; Macrophage mannose receptor 1-like protein 1; CD206; MRC1; CLEC13D; CLEC13DL; MRC1L1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human Mannose Receptor/MRC1 recombinant protein (Position: D21-A1140). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Mannose Receptor/MRC1 Picoband® Antibody is an antibody for MRC1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MRC1 (mannose receptor C-type 1); UniProt: P22897; NCBI Gene: 4360
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 190-200 kDa, calculated 75694 MW
- Applications: WB, IHC, IF, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Mannose Receptor/MRC1 Picoband® Antibody catalog # A02285-2.
Biological background
Biological context: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. (Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E. (Microbial infection) Interacts with Hepatitis B virus envelope protein.
Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein. Endosome membrane. Single-pass type I membrane protein., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare MRC1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MRC1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify MRC1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass type I membrane protein. Endosome membrane. Single-pass type I membrane protein.
- Tissue details: Ubiquitous.
- Research category: Cardiovascular,Cell Biology,Cell Cycle,Cell Differentiation,Lipases,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
