| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage colony-stimulating factor 1;CSF-1;MCSF;Processed macrophage colony-stimulating factor 1;Csf1;Csfm; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes macrophage colony-stimulating factor 1 (gene Csf1) in mouse and rat samples and is validated for ELISA and IHC. The predicted molecular weight is 60.6 kDa.
M-CSF (CSF1) is a cytokine that supports the survival, proliferation and differentiation of monocytes, macrophages and their precursors by signaling through the CSF1R receptor. It is also needed for osteoclast formation and bone resorption, and influences inflammation, fertility and cell migration, so it is measured in studies of macrophage biology, bone remodeling and inflammatory disease.
| Target | Macrophage colony-stimulating factor 1 (gene Csf1; UniProt P07141, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 60.6 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse MCSF fragment (Lys33–Glu262), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places macrophage colony-stimulating factor 1 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.