| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Macrophage colony-stimulating factor 1;CSF-1;MCSF;Processed macrophage colony-stimulating factor 1;Csf1;Csfm; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Csf1 (Macrophage colony-stimulating factor 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-MCSF/CSF1 Antibody Picoband® catalog # PB9562. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively. (reported region: K33-E262).
- Molecular weight context: reported MW: 130 kDa; calculated MW: 60649 MW
- Reactivity: Mouse,Rat
- Applications: ELISA, IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Functional note: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Reported localization: Cell membrane ; Single-pass type I membrane protein . Expression/tissue context: Non-neural epithelial tissues.
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how Csf1 (Macrophage colony-stimulating factor 1) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how Csf1 (Macrophage colony-stimulating factor 1) relates to this theme using model systems and orthogonal readouts.
- Cytokines: Researchers commonly examine how Csf1 (Macrophage colony-stimulating factor 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of Csf1 (Macrophage colony-stimulating factor 1) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 5 cadherin domains.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
