| Field | Specification |
|---|---|
| Target | |
| Alternative names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1; PDHK1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PDK1 recombinant protein (Position: V107-Y336). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (gene PDK1) in human and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 49.2 kDa, and the supplier reports an observed band at about 58 kDa.
Pyruvate dehydrogenase kinase 1 (PDK1) is a mitochondrial kinase that phosphorylates the PDHA subunit and switches off the pyruvate dehydrogenase complex, reducing conversion of pyruvate to acetyl-CoA. It is a direct HIF-1 target that shifts cells toward glycolysis under hypoxia, and it is studied in cancer metabolism. It should not be confused with PDPK1, the AGC kinase also called PDK1.
| Target | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (gene PDK1; UniProt Q15118, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 49.2 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human PDK1 fragment (Val107–Tyr336), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed predominantly in the heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiong Liu et al. (2022) Identification of PIK3CG as a hub in septic myocardial injury using network pharmacology and weighted gene co-expression network analysis. Bioengineering & Translational Medicine. 10.1002/btm2.10384
Xu K et al. (2009) Silencing of HIF-1α suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis. Cancer Gene Therapy. 10.1038/cgt.2009.66
