| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagenase 3;3.4.24.-;Matrix metalloproteinase-13;MMP-13;MMP13; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MMP13 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IDA-13 is a rabbit monoclonal antibody that recognizes collagenase 3 (gene MMP13) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 53.8 kDa, and the supplier reports an observed band at about 60 kDa.
Collagenase 3 (MMP-13) is a matrix metalloproteinase that degrades fibrillar collagens, with the highest activity toward type II collagen of cartilage, and it also cleaves aggrecan and other matrix proteins. It is needed for normal bone development and is widely studied in cartilage breakdown in osteoarthritis.
| Target | Collagenase 3 (gene MMP13; UniProt P45452, human) |
|---|---|
| Clone | IDA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.8 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human MMP13.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes collagenase 3 as a secreted protein. Tissue expression noted by UniProt (human): Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.