Anti-MMP13 Rabbit Monoclonal Antibody

SKU:BHA21008166
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-MMP13 antibody (Rabbit monoclonal, clone IDA-13, Rabbit IgG). Validated for Western blot. Reactivity: Human, Mouse. Recognizes collagenase 3. Observed band in Western blot: about 60 kDa.
Target MMP13
Clone number IDA-13
Host Rabbit
Reactivity Human,Mouse
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M00420 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB
      Application details: WB 1:500-2000
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MMP13
Alternative names Collagenase 3;3.4.24.-;Matrix metalloproteinase-13;MMP-13;MMP13;
UniProt # P45452
Host Rabbit
Clone IDA-13
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human MMP13
Molecular weight 53.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted, extracellular space, extracellular matrix . Secreted .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00420
Main SKU BHA21008166
Rabbit Monoclonal

Product Overview

Clone IDA-13 is a rabbit monoclonal antibody that recognizes collagenase 3 (gene MMP13) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 53.8 kDa, and the supplier reports an observed band at about 60 kDa.

Collagenase 3 (MMP-13) is a matrix metalloproteinase that degrades fibrillar collagens, with the highest activity toward type II collagen of cartilage, and it also cleaves aggrecan and other matrix proteins. It is needed for normal bone development and is widely studied in cartilage breakdown in osteoarthritis.

Target Collagenase 3 (gene MMP13; UniProt P45452, human)
Clone IDA-13
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 53.8 kDa
Observed MW 60 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human MMP13.

Reactivity Notes

The supplier lists reactivity with human and mouse. UniProt describes collagenase 3 as a secreted protein. Tissue expression noted by UniProt (human): Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 53.8 kDa; observed band at about 60 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human MMP13.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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