| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromelysin-1;SL-1;3.4.24.17;Matrix metalloproteinase-3;MMP-3;Transin-1;MMP3;STMY1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human MMP3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by ten amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets stromelysin-1 (gene MMP3) in human samples and is validated for Western blot. The predicted molecular weight is 54.0 kDa, and the supplier reports an observed band at about 22–24 kDa.
Stromelysin-1 (MMP-3) is a matrix metalloproteinase with broad specificity that degrades fibronectin, laminin, proteoglycans and several collagens. It also activates other MMPs such as pro-MMP-9, and it is widely studied in arthritis, tissue remodeling and neuroinflammation.
| Target | Stromelysin-1 (gene MMP3; UniProt P08254, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.0 kDa |
| Observed MW | 22–24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue, U2OS cells, HeLa cells, PANC cells, COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of at the C-terminal of human MMP3. It differs from the mouse sequence at seven residues and from the rat sequence at ten residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places stromelysin-1 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Quiñones-Díaz Blanca I. et al. (2020) MicroRNA-18a-5p Suppresses Tumor Growth via Targeting Matrix Metalloproteinase-3 in Cisplatin-Resistant Ovarian Cancer. Frontiers in Oncology. 10.3389/fonc.2020.602670
Yi Shen et al. (2025) Monotropein synergizes with methotrexate to attenuate synovial inflammation in adjuvant-induced arthritis mice and fibroblast-like synoviocyte via GSK-3β. International Immunopharmacology. 10.1016/j.intimp.2025.114817
Weibiao Xiong et al. (2022) Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis. Cancer Management and Research. 10.2147/CMAR.S302084
Wei Jia et al. (2018) Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. International Journal of Oncology. 10.3892/ijo.2018.4330
Zhai Ke-feng et al. (2017) Protective effects of paeonol on inflammatory response in IL-1β-induced human fibroblast-like synoviocytes and rheumatoid arthritis progression via modulating NF-κB pathway. Inflammopharmacology. 10.1007/s10787-017-0385-5
