| Field | Specification |
|---|---|
| Target | |
| Alternative names | 55 kDa erythrocyte membrane protein;p55;Membrane protein, palmitoylated 1;MPP1;DXS552E, EMP55; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MPP1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 55 kDa erythrocyte membrane protein (gene MPP1) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 55 kDa.
MPP1 (p55) is a palmitoylated membrane protein of the MAGUK family, first identified in red blood cells, where it links the membrane skeleton to the plasma membrane. It also regulates neutrophil polarity, and it is studied in membrane organization and cell polarity.
| Target | 55 kDa erythrocyte membrane protein (gene MPP1; UniProt Q00013, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 52.3 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
Samples with a confirmed band (WB): rat lung tissue, mouse spleen tissue, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Immunogen
Synthetic peptide from the C-terminal region of human MPP1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 55 kDa erythrocyte membrane protein in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yingli Chen et al. (2020) Inositol-requiring enzyme 1α links palmitate-induced mTOR activation and lipotoxicity in hepatocytes. American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00165.2020
