| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-related protein M-Ras; Ras-related protein R-Ras3; MRAS; RRAS3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Mras |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29M72 is a rabbit monoclonal antibody that recognizes ras-related protein M-Ras (gene MRAS) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.8 kDa, and the supplier reports an observed band at about 24 kDa.
M-Ras (MRAS) is a Ras-family small GTPase that activates the MAPK pathway. In its GTP-bound form it forms the SHOC2-MRAS-PP1c complex, which removes an inhibitory phosphate from RAF kinases to promote their activation, and MRAS mutations are linked to Noonan syndrome.
| Target | Ras-related protein M-Ras (gene MRAS; UniProt O14807, human) |
|---|---|
| Clone | 29M72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.8 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human mras.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein M-Ras in the cell membrane. Tissue expression noted by UniProt (human): Expression highly restricted to the brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
