| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human MUC5AC |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MUC5AC antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABAD-13; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody catalog # M00612-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MUC5AC (Mucin-5AC).
- Antibody format: Monoclonal; clone ABAD-13; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MUC5AC (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. Reported cellular localization context: Secreted . Tissue expression notes (as provided): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Research relevance and current trends
- Research context keywords from the source record include: Signal Transduction,Cancer,Cytoskeleton/ECM,Extracellular Matrix,Tumor Biomarkers,Invasion/Microenvironment,ECM Proteins,Tumor Antigens,Tumor Immunology,Tumor-Associated Antigens.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate MUC5AC antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MUC5AC expression by Western blot in cell or tissue lysates, Detect MUC5AC in FFPE tissue sections by immunohistochemistry, Localize MUC5AC by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 32-42 kDa, 65 kDa, 75 kDa; calculated MW: 585570 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 32-42 kDa, 65 kDa, 75 kDa
- Cellular localization (provided): Secreted .
- Tissue details (provided): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
