| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUC5AC |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABAD-13 is a rabbit monoclonal antibody that recognizes mucin-5AC (gene MUC5AC) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 585.6 kDa, and the supplier reports an observed band at about 220–600 kDa.
Mucin-5AC (MUC5AC) is a major gel-forming mucin secreted by goblet cells of the airways and surface cells of the stomach. It traps inhaled particles and microbes for mucociliary clearance and protects the gastric mucosa. MUC5AC is a common marker in research on airway mucus hypersecretion, asthma, COPD and gastric epithelial biology.
| Target | Mucin-5AC (gene MUC5AC; UniProt P98088, human) |
|---|---|
| Clone | ABAD-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 585.6 kDa |
| Observed MW | 220–600 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human stomach.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MUC5AC.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes mucin-5AC as a secreted protein. Tissue expression noted by UniProt (human): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Elisa Danese et al. (2023) Plasma Bile Acid Profiling and Modulation of Secreted Mucin 5AC in Cholangiocarcinoma. International Journal of Molecular Sciences. 10.3390/ijms241612794
