| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein 105 kDa;Antigen NY-CO-25;Heat shock 110 kDa protein;HSPH1;HSP105, HSP110, KIAA0201; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MYBBP1A May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity) . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
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Overview
This product is an anti-MYBBP1A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADIE-13; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-MYBBP1A Monoclonal Antibody catalog # M04187. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MYBBP1A (Heat shock protein 105 kDa).
- Antibody format: Monoclonal; clone ADIE-13; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MYBBP1A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity). . Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Co-Factors,Epigenetics and Nuclear Signaling,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate MYBBP1A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MYBBP1A expression by Western blot in cell or tissue lysates, Detect MYBBP1A in FFPE tissue sections by immunohistochemistry, Localize MYBBP1A by immunofluorescence/immunocytochemistry in cultured cells, Quantify MYBBP1A-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 51 kDa; calculated MW: 96865 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 51 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
