| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase;Myeloperoxidase light chain;Myeloperoxidase heavy chain;MPO; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Myeloperoxidase |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MPO antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOCF-13; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody catalog # M00372. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MPO (Myeloperoxidase).
- Antibody format: Monoclonal; clone AOCF-13; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MPO (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Reported cellular localization context: Lysosome. Tissue expression notes (as provided): Highly expressed in substantia nigra. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Metabolism,Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Pathways and Processes,Redox Metabolism,Vasculature.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate MPO antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MPO expression by Western blot in cell or tissue lysates, Detect MPO in FFPE tissue sections by immunohistochemistry, Localize MPO by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 84 kDa; calculated MW: 83869 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 84 kDa
- Cellular localization (provided): Lysosome.
- Tissue details (provided): Highly expressed in substantia nigra. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
