| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase;Myeloperoxidase light chain;Myeloperoxidase heavy chain;MPO; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Myeloperoxidase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOCF-13 is a rabbit monoclonal antibody that recognizes myeloperoxidase (gene MPO) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 83.9 kDa, and the supplier reports an observed band at about 84 kDa.
Myeloperoxidase (MPO) is a heme peroxidase stored in neutrophil granules. It uses hydrogen peroxide to generate hypochlorous acid and other oxidants that kill microbes, and it also helps decondense chromatin during neutrophil extracellular trap (NET) formation, so it is a standard neutrophil marker in inflammation research.
| Target | Myeloperoxidase (gene MPO; UniProt P05164, human) |
|---|---|
| Clone | AOCF-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.9 kDa |
| Observed MW | 84 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HL-60 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human myeloperoxidase.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes myeloperoxidase as secreted and also located in the lysosome, cytolytic granule and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Specifically expressed in neutrophils and monocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaolin Ma et al. (2025) Paeonol inhibits NETs-mediated foam cell inflammation through the CitH3/NLRP3/caspase-1 signaling pathway in atherosclerosis. International Immunopharmacology. 10.1016/j.intimp.2025.114340
