Anti-Myosin(Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32)

SKU:BHA21006538
Research Validated
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Boster Bio
Boster Bio
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Overview
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Anti-MYH1; MYH2 antibody (Mouse monoclonal, clone MY-32, Mouse IgG1). Validated for Western blot and immunohistochemistry. Reactivity: Human, Mouse, Rabbit, Rat. Recognizes myosin-2. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target MYH1; MYH2
Clone number MY-32
Host Mouse
Reactivity Human,Mouse,Rabbit,Rat
Isotype Mouse IgG1
Application(s) WB, IHC
Options selector
Catalog no. Size Conjugation
MA1063 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: IHC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 1.5-2μg/ml, Human, mouse, rabbit, rat
      Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MYH1; MYH2
Alternative names Myh2 protein ;Myh2 ;
Gene ID Human(4619), Mouse(17879), Rat(287408), Human(4620), Mouse(17882), Rat(691644)
UniProt # I6L963
Host Mouse
Clone MY-32
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen Rabbit muscle myosin.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1063
Main SKU BHA21006538
Mouse Monoclonal

Product Overview

Clone MY-32 is a mouse monoclonal antibody that recognizes myosin-2 (gene Myh2) in human, mouse, rabbit and rat samples and is validated for Western blot and IHC.

Myosin-2 (MYH2) is the skeletal muscle myosin heavy chain that defines fast type IIa fibers. As the motor of the thick filament it hydrolyzes ATP to drive muscle contraction, and antibodies to it are used to type muscle fibers in studies of exercise, aging and myopathy.

Target Myosin-2 (gene Myh2; UniProt I6L963, mouse)
Clone MY-32
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Human, Mouse, Rabbit, Rat
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 1.5–2 µg/mL (Human, Mouse, Rabbit, Rat)
Western blot 1–2 µg/mL (Human, Mouse, Rabbit, Rat)

Immunogen

Rabbit muscle myosin.

Reactivity Notes

The supplier lists reactivity with human, mouse, rabbit and rat. UniProt places myosin-2 in the cytoplasm.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1–2 µg/mL (Human, Mouse, Rabbit, Rat); IHC (paraffin sections) 1.5–2 µg/mL (Human, Mouse, Rabbit, Rat). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rabbit, Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Rabbit muscle myosin.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Olatunji Ajiteru et al. (2021) A digital light processing 3D printed magnetic bioreactor system using silk magnetic bioink. Biofabrication. 10.1088/1758-5090/abfaee

Sun Qiang et al. (2008) Transforming growth factor-β-regulated miR-24 promotes skeletal muscle differentiation. Nucleic Acids Research. 10.1093/nar/gkn032

Huaqin Li et al. (2019) PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway. Cells. 10.3390/cells8090959

Mei Wang et al. (2023) Correction of Glycogen Synthase Kinase 3β in Myotonic Dystrophy 1 Reduces the Mutant RNA and Improves Postnatal Survival of DMSXL Mice. Molecular and Cellular Biology. 10.1128/MCB.00155-19

Sun M et al. (2015) Autoregulatory loop between TGF-β1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation. Cell Death & Disease. 10.1038/cddis.2015.225

Zijian Ye et al. (2020) MiR-92b-3p inhibits proliferation and migration of C2C12 cells. Cell Cycle. 10.1080/15384101.2020.1827511

Cao Ximei et al. (2022) Analyses of the circadian clock genes expression in whole embryos and maternal major tissues of mice. Journal of Molecular Histology. 10.1007/s10735-022-10065-x

Qiu Guoying et al. (2013) AngII-induced glomerular mesangial cell proliferation inhibited by losartan via changes in intracellular calcium ion concentration. Clinical and Experimental Medicine. 10.1007/s10238-013-0232-y

Kai Dang et al. (2016) Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). Biology Open. 10.1242/bio.015776

Yong Wei Nie et al. (2013) Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5. Cell Biology International. 10.1002/cbin.10124

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