| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Myh2 protein ;Myh2 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Rabbit muscle myosin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Myosin(Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32) is an antibody targeting MYH1; MYH2. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: MY-32; isotype: Mouse IgG1; reactivity: Human,Mouse,Rabbit,Rat; observed MW: 200 kDa; calculated MW: 183084 MW.
Boster Bio Anti-Myosin (Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32) catalog # MA1063. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Key elements and design rationale
- Target: MYH1; MYH2 — myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: MY-32; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rabbit,Rat
- Molecular weight guidance: Observed: 200 kDa; Calculated: 183084 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): MYH1: Muscle contraction.|MYH2: Muscle contraction. Required for cytoskeletonorganization (By similarity).
Scientific background (datasheet): Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types (MYL1 according to the HGM symbols). Skeletal Myosin (Fast),, also known as MYL1, mapped to the region 2q32.1-qter.
Cellular localization (datasheet): MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.
Sequence similarities (datasheet): Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Research relevance and current trends
- Commonly studied in contexts related to Cardiovascular,Contractility,Cytoskeleton,Cytoskeleton/ECM,Motor Proteins,Myosin,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
