| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chemokine (C-X-C motif) ligand 7, isoform CRA_b ;Chemokine subfamily B Cys-X-Cys ;Platelet basic protein ;Pro-platelet basic protein ;Putative uncharacterized protein ;Thymus chemokine 1 ;Ppbp ;Cxcl7 ;mCG_1702 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse NAP2 recombinant protein (Position: K40-Y113). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects chemokine subfamily B Cys-X-Cys (gene Ppbp) in mouse samples and is validated for IHC and Western blot.
Pro-platelet basic protein (PPBP) is stored in platelet granules and released on activation. It is cleaved into several peptides, including connective tissue-activating peptide III, β-thromboglobulin and the chemokine NAP-2 (CXCL7). NAP-2 signals through CXCR1 and CXCR2 to attract neutrophils, so PPBP is studied in platelet activation and inflammation.
| Target | Chemokine subfamily B Cys-X-Cys (gene Ppbp; UniProt Q9EQI5, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): mouse bone tissue, mouse lung tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse NAP2 fragment (Lys40–Tyr113), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes chemokine subfamily B Cys-X-Cys as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
