| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;NEFH;KIAA0845, NFH; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NEFH |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOEO-14 is a rabbit monoclonal antibody that recognizes neurofilament heavy polypeptide (gene NEFH) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 112.5 kDa, and the supplier reports an observed band at about 200 kDa.
Neurofilament heavy chain (NF-H, NEFH) is one of the three subunits of neurofilaments, the intermediate filaments of neurons, together with NF-L and NF-M. Its long, heavily phosphorylated tail is abundant in mature axons and helps set axon caliber, and NF-H antibodies are common markers of neurons and axons.
| Target | Neurofilament heavy polypeptide (gene NEFH; UniProt P12036, human) |
|---|---|
| Clone | AOEO-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.5 kDa |
| Observed MW | 200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NEFH.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neurofilament heavy polypeptide in the cytoplasm and cell projection. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bo Wang et al. (2022) Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage. Oxidative Medicine and Cellular Longevity. 10.1155/2022/6160701
Chen Chen et al. (2018) Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury. Neural Regeneration Research. 10.4103/1673-5374.233449
