| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nephrin;Renal glomerulus-specific cell adhesion receptor;NPHS1;NPHN; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nephrin (gene NPHS1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 134.7 kDa, and the supplier reports an observed band at about 185 kDa.
Nephrin (NPHS1) is an immunoglobulin-superfamily adhesion protein of kidney podocytes and a central structural component of the slit diaphragm between podocyte foot processes. It links the slit diaphragm to the actin cytoskeleton and signals to maintain the filtration barrier. Mutations in NPHS1 cause congenital nephrotic syndrome of the Finnish type.
| Target | Nephrin (gene NPHS1; UniProt O60500, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 134.7 kDa |
| Observed MW | 185 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Nephrin fragment (Val1084–Val1241), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 79.9% and 75.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nephrin in the cell membrane. Tissue expression noted by UniProt (human): Specifically expressed in podocytes of kidney glomeruli. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shuhui Zhou et al. (2024) Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels. Frontiers in Pharmacology. 10.3389/fphar.2024.1447249
Chen Yueqi et al. (2024) Exosomal miR-223-3p from bone marrow mesenchymal stem cells targets HDAC2 to downregulate STAT3 phosphorylation to alleviate HBx-induced ferroptosis in podocytes. Frontiers in Pharmacology. 10.3389/fphar.2024.1327149
Hui Liao et al. (2020) Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation. Journal of Immunology Research. 10.1155/2020/8263598
