| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Neuronal Pentraxin-2, NP2, Neuronal Activity-Regulated Pentaxin, NARP |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-Neuronal Pentraxin 2 (NPTX2) Antibody is an antibody targeting Neuronal Pentraxin-2, NP2, Neuronal Activity-Regulated Pentaxin, NARP Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Neuronal Pentraxin-2, NP2, Neuronal Activity-Regulated Pentaxin, NARP (also reported as Neuronal Pentraxin-2, NP2, Neuronal Activity-Regulated Pentaxin, NARP).
- Immunogen/epitope region: Secreted.
- Homology note: Human - identical, rat - 12 out of 13 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Neuronal pentraxin 2 (NP2), also known as NPTX2, NP-II, and neuronal activity-regulated pentraxin (Narp), is a calcium-dependent lectin secreted protein member of the pentraxin family involved in synaptic plasticity and excitatory homeostasis.1,2NP2 is part of the neuronal pentraxin family that includes two other members: Neuronal Pentraxin 1 (NP1) and Neuronal Pentraxin Receptor (NPTXR). The pentraxin superfamily members are arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain 2,3.NP2 is widely expressed in the brain, especially in the hippocampus, dentate gyrus, cerebellum, and cerebral cortex regions, as well as in the testis, pancreas, liver, heart, and skeletal muscle.2,3NP2 interacts with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, which are crucial for excitatory neurotransmission in the brain.
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR192.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR192; Negative control: BLP-NR192.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
