| Field | Specification |
|---|---|
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| Accession Number | |
| Alternative Names | NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody is an antibody targeting NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022 (also reported as NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022).
- Immunogen/epitope region: Extracellular, 2nd loop..
- Homology note: Rat - identical Human - 13 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Neuropeptide FF receptor 1, NPFFR1, also known as GPR147 and RFamide-related peptide receptor 0T7T022, is a Gi/o protein-coupled receptor that mainly couples to Gαi3 and Gαs proteins and binds NPFF and RF-amide related peptides that are characterized by a conserved C-terminal RF-amide motif.1,2NPFF has been identified in various species, including humans, rodents, bovines, and guinea pigs, indicating its physiological significance. NPFF has been detected in discrete areas of the rat central nervous system (CNS), with the highest levels observed in the dorsal spinal cord and the posterior lobe of the pituitary gland. The distribution of NPFF mRNA at the tissue level generally corresponds with NPFF immunoreactivity, except in the hypothalamic region where differences have been observed.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR073.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR073; Negative control: BLP-NR073.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
