| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-neuropeptide Y;Neuropeptide Y;Neuropeptide tyrosine;NPY;C-flanking peptide of NPY;CPON;NPY; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Neuropeptide Y, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pro-neuropeptide Y (gene NPY) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 10.9 kDa, and the supplier reports an observed band at about 11 kDa.
Neuropeptide Y (NPY) is a 36-amino-acid peptide and one of the most abundant neuropeptides in the brain, also released by sympathetic neurons. Acting through Y-family G protein-coupled receptors, it strongly stimulates food intake and also affects stress, anxiety, circadian rhythms and blood vessel tone. NPY is widely studied in energy balance and neuroscience.
| Target | Pro-neuropeptide Y (gene NPY; UniProt P01303, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 10.9 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with confirmed staining (IHC): human brain tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human neuropeptide Y. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes pro-neuropeptide Y as a secreted protein. Tissue expression noted by UniProt (human): One of the most abundant peptides in the nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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