| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pro-neuropeptide Y;Neuropeptide Y;Neuropeptide tyrosine;NPY;C-flanking peptide of NPY;CPON;NPY; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Neuropeptide Y, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NPY (Pro-neuropeptide Y) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Neuropeptide Y/NPY Antibody Picoband® catalog # PB9296. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human Neuropeptide Y, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 11 kDa; calculated MW: 10851 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Pro-neuropeptide Y; Pro-neuropeptide Y. This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. Functional note: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Reported localization: Secreted. Expression/tissue context: One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Research relevance and current trends
- Biochemicals: Researchers commonly examine how NPY (Pro-neuropeptide Y) relates to this theme using model systems and orthogonal readouts.
- Chemical Type: Researchers commonly examine how NPY (Pro-neuropeptide Y) relates to this theme using model systems and orthogonal readouts.
- Neuropeptides: Researchers commonly examine how NPY (Pro-neuropeptide Y) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NPY (Pro-neuropeptide Y) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of NPY (Pro-neuropeptide Y) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the NPY family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
