| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-13;IL-13;IL13;NC30; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NFE2L2 recombinant protein (Position: R34-N605). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NFE2L2 Antibody Picoband® is an antibody for NFE2L2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NFE2L2 (Interleukin-13); UniProt: Q16236
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 100 kDa, calculated 15816 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NFE2L2 Antibody Picoband® catalog # A00078-1.
Biological background
Biological context: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
Expression and localization notes: cellular localization: Secreted., tissue context: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads..
Common research applications
- Western blotting (WB): Compare NFE2L2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NFE2L2 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify NFE2L2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: NFE2L2 (nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-ed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas (G12D) and BRaf (V619E), and in human pancreatic cancer.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted.
- Tissue details: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
- Research category: Cytokines,Immunology,Innate Immunity,Interleukins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
