| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurogenic locus notch homolog protein 4;Notch 4;hNotch4;Notch 4 extracellular truncation;Notch 4 intracellular domain;NOTCH4;INT3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NOTCH4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects neurogenic locus notch homolog protein 4 (gene NOTCH4) in human samples and is validated for Western blot. The predicted molecular weight is 209.6 kDa, and the supplier reports an observed band at about 210 kDa.
NOTCH4 is a Notch family receptor expressed mainly in endothelial cells. After ligand binding, its intracellular domain is released and acts with RBPJ to regulate transcription. It is studied in vascular development and angiogenesis and was originally identified as the mouse Int-3 mammary oncogene.
| Target | Neurogenic locus notch homolog protein 4 (gene NOTCH4; UniProt Q99466, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 209.6 kDa |
| Observed MW | 210 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): A549 cells, SMMC cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human NOTCH4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neurogenic locus notch homolog protein 4 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao-Ni YANG et al. (2017) Protective effect of Shouwu Yizhi decoction against vascular dementia by promoting angiogenesis. Chinese Journal of Natural Medicines. 10.1016/S1875-5364(17)30105-X
