| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein NOV homolog;NovH;CCN family member 3;Insulin-like growth factor-binding protein 9;IBP-9;IGF-binding protein 9;IGFBP-9;Nephroblastoma-overexpressed gene protein homolog;NOV;CCN3, IGFBP9, NOVH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NOV (AP-2 complex subunit mu) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-NOV/CCN3 Antibody Picoband® catalog # A06319-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.
- Molecular weight context: reported MW: 47 kDa; calculated MW: 39162 MW
- Reactivity: Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
AP-2 complex subunit mu; Protein NOV homolog. NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. Functional note: Immediate-early protein likely to play a role in cell growth regulation. . Reported localization: Secreted. Expression/tissue context: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .
Research relevance and current trends
- DNA/RNA: Researchers commonly examine how NOV (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how NOV (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
- RNA Processing: Researchers commonly examine how NOV (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NOV (AP-2 complex subunit mu) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of NOV (AP-2 complex subunit mu) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
