| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADPH oxidase activator 1;NOX activator 1;Antigen NY-CO-31;NCF2-like protein;P67phox-like factor;p51-nox;NOXA1;P51NOX; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NOXA1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NADPH oxidase activator 1 (gene NOXA1) in human samples and is validated for Western blot. The predicted molecular weight is 50.9 kDa, and the supplier reports an observed band at about 60 kDa.
NOX activator 1 (NOXA1) is a cytosolic regulatory protein related to p67phox that switches on NOX1 to produce superoxide. It can also support NOX3 and NOX2 activity. NOXA1 is studied in reactive oxygen species signaling in colon epithelium and blood vessels.
| Target | NADPH oxidase activator 1 (gene NOXA1; UniProt Q86UR1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 50.9 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human U87 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human NOXA1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NADPH oxidase activator 1 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yezhou Liu et al. (2023) A predicted structure of NADPH Oxidase 1 identifies key components of ROS generation and strategies for inhibition. PLoS One. 10.1371/journal.pone.0285206
