| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-NPBWR1 Antibody is an antibody targeting Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7 (also reported as Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7).
- Immunogen/epitope region: Intracellular, 2nd loop..
- Homology note: Rat - identical; human - 12 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Specificity statement (as provided): The antibody is specific for NPBWR1, it will not recognize the human only receptor NPBWR2..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Neuropeptides B/W receptor type 1 (NPBWR1), also known as G-protein coupled receptor 7 (GPR7), is a member of the G-protein coupled receptor (GPCR) superfamily.NPBWR1 mediates the biological activities of Neuropeptide B (NPB) and neuropeptide W (NPW) that are structurally and functionally related endogenous neuropeptides. In humans, a second receptor termed NPBWR2 or GPR8, has been identified, however, NPBWR2 is absent in rodents.1,2NPBWR1 is expressed in the central nervous system (CNS) and in peripheral tissues. In the brain expression of NPBWR1 is found in several regions including hypothalamus, hippocampus and amygdala.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR075.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR075; Negative control: BLP-NR075.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
