| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bactericidal permeability-increasing protein;BPI;CAP 57;BPI; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human NPHS2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NPHS2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18N90; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-NPHS2 Rabbit Monoclonal Antibody catalog # M01445-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: NPHS2 (Bactericidal permeability-increasing protein).
- Antibody format: Monoclonal; clone 18N90; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NPHS2 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. . Reported cellular localization context: Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. . Tissue expression notes (as provided): Restricted to cells of the myeloid series.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cell Biology,Cell Death,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Metabolism Processes,Pathways and Processes,T Cells.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate NPHS2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NPHS2 expression by Western blot in cell or tissue lysates, Detect NPHS2 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 33 kDa; calculated MW: 53900 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 33 kDa
- Cellular localization (provided): Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. .
- Tissue details (provided): Restricted to cells of the myeloid series.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
