| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NR0B1 / Dax1 Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This product is an anti-NR0B1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AECC-14; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-NR0B1 / DAX1 Monoclonal Antibody catalog # M01521. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: NR0B1 (Macrophage metalloelastase).
- Antibody format: Monoclonal; clone AECC-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NR0B1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Reported cellular localization context: Secreted, extracellular space, extracellular matrix . Tissue expression notes (as provided): Found in alveolar macrophages but not in peripheral blood monocytes.
Research relevance and current trends
- Research context keywords from the source record include: Developmental Biology,Endocrine System,Epigenetics and Nuclear Signaling,Neuroscience,Nuclear Receptors,Nuclear Signaling Pathways,Reproduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate NR0B1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NR0B1 expression by Western blot in cell or tissue lysates, Detect NR0B1 in FFPE tissue sections by immunohistochemistry, Localize NR0B1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify NR0B1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 54002 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Secreted, extracellular space, extracellular matrix .
- Tissue details (provided): Found in alveolar macrophages but not in peripheral blood monocytes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
