| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor erythroid 2-related factor 2;NF-E2-related factor 2;NFE2-related factor 2;HEBP1;Nuclear factor, erythroid derived 2, like 2;NFE2L2;NRF2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nrf2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AGH-14 is a rabbit monoclonal antibody that recognizes nuclear factor erythroid 2-related factor 2 (gene NFE2L2) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 67.8 kDa, and the supplier reports an observed band at about 90–110 kDa.
NRF2 (NFE2L2) is a CNC-bZIP transcription factor that controls the cellular antioxidant response. Under normal conditions KEAP1 targets it for degradation, but oxidative or electrophilic stress stabilizes NRF2, which then binds antioxidant response elements to induce detoxifying and cytoprotective genes, making it central to redox biology.
| Target | Nuclear factor erythroid 2-related factor 2 (gene NFE2L2; UniProt Q16236, human) |
|---|---|
| Clone | AGH-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 67.8 kDa |
| Observed MW | 90–110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:100–1:500 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human nrf2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places nuclear factor erythroid 2-related factor 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
