| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2; CDKN1C; KIP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Occludin/OCLN recombinant protein (Position: H54-K270). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Anti-Occludin/OCLN Antibody Picoband® is an antibody for OCLN detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: OCLN (cyclin dependent kinase inhibitor 1C); UniProt: Q16625
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 65 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Occludin/OCLN Antibody Picoband® catalog # A01246-2.
Biological background
Biological context: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver..
Common research applications
- Western blotting (WB): Compare OCLN levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of OCLN in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify OCLN-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.
- Research category: Adaptive Immunity,Angiogenesis,Atherosclerosis,Cardiovascular,Cell Adhesion Molecules,Cell Type Markers,Hematopoietic Progenitors,Immunology,Myeloid,Platelets,Stem Cells,Vascular Inflammation
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
