| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Occludin;OCLN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Occludin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-OCLN antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone IEH-15; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-Occludin OCLN Rabbit Monoclonal Antibody catalog # M01246. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: OCLN (Occludin).
- Antibody format: Monoclonal; clone IEH-15; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
OCLN (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. . Reported cellular localization context: Membrane; Multi-pass membrane protein. Cell junction, tight junction. Tissue expression notes (as provided): Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Research relevance and current trends
- Research context keywords from the source record include: Cell Adhesion,Cytoskeleton/ECM,Signal Transduction,Tight Junctions.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate OCLN antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect OCLN expression by Western blot in cell or tissue lysates, Quantify OCLN-positive cells by flow cytometry in single-cell suspensions, Enrich OCLN by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 59144 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 80 kDa
- Cellular localization (provided): Membrane; Multi-pass membrane protein. Cell junction, tight junction.
- Tissue details (provided): Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
