| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase inhibitor 1;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;CDKN1A;CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human p21 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAI-3 is a rabbit monoclonal antibody that recognizes cyclin-dependent kinase inhibitor 1 (gene CDKN1A) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 18.1 kDa, and the supplier reports an observed band at about 21 kDa.
p21 (CDKN1A, also called p21Cip1/WAF1) is a cyclin-dependent kinase inhibitor that halts the cell cycle, mainly in G1 and G2. Its expression is strongly induced by p53 after DNA damage, and it is widely used as a marker of cell cycle arrest, DNA damage responses and cellular senescence.
| Target | Cyclin-dependent kinase inhibitor 1 (gene CDKN1A; UniProt P38936, human) |
|---|---|
| Clone | EAI-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.1 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human p21.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase inhibitor 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in all adult tissues, with 5-fold lower levels observed in the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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