Anti-P2X7/P2RX7 Antibody Picoband®

Overview

Anti-P2X7/P2RX7 Antibody Picoband® is an antibody for P2RX7 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: P2RX7 (vinculin); UniProt: Q99572
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 70 kDa, calculated 123799 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-P2X7/P2RX7 Antibody Picoband® catalog # A01208-3.

Biological background

Biological context: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Expression and localization notes: cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Sarcolemma. Cytoskeleton. Adherens junction. Focal adhesion., tissue context: Metavinculin is muscle-specific..

Common research applications

• Western blotting (WB): Compare P2RX7 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of P2RX7 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify P2RX7-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Sarcolemma. Cytoskeleton. Adherens junction. Focal adhesion.
• Tissue details: Metavinculin is muscle-specific.
• Research category: Actin Binding Proteins,Actin, etc.,Cardiovascular,Contractility,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.