| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Claudin-1;Senescence-associated epithelial membrane protein;CLDN1;CLD1, SEMP1;UNQ481/PRO944; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Parathyroid Hormone Receptor 1 Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PTH1R antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEHO-16; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-Parathyroid Hormone Receptor 1 Monoclonal Antibody catalog # M01588. Tested in WB, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: PTH1R (Claudin-1).
- Antibody format: Monoclonal; clone AEHO-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PTH1R (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an in effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391). . Reported cellular localization context: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. .
Research relevance and current trends
- Research context keywords from the source record include: Endocrine System,Growth Factors/Hormones,Neuroscience,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PTH1R antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PTH1R expression by Western blot in cell or tissue lysates, Enrich PTH1R by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 47 kDa; calculated MW: 22744 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 47 kDa
- Cellular localization (provided): Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
- Tissue details (provided): Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
