| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD (+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;PARP1;ADPRT, PPOL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human PARP |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PARP PARP1 Rabbit Monoclonal Antibody is an antibody targeting PARP1. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CFD-16; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 70 kDa; calculated MW: 113084 MW.
Boster Bio Anti-PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PARP1 — Poly [ADP-ribose] polymerase 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CFD-16; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 70 kDa; Calculated: 113084 MW
Biological background
Protein function (datasheet): Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to ly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. .
Cellular localization (datasheet): Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
Tissue details (datasheet): Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .
Research relevance and current trends
- Commonly studied in contexts related to Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
