| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PD-L1 (CD274) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IDC-3 is a rabbit monoclonal antibody that recognizes programmed cell death 1 ligand 1 (gene CD274) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 33.3 kDa, and the supplier reports an observed band at about 40 kDa.
PD-L1 (CD274) is a transmembrane ligand that binds the inhibitory receptor PD-1 on T cells and limits their activation, helping maintain tolerance to self. Many tumors upregulate PD-L1 to escape immune attack, which made the PD-1/PD-L1 axis a cornerstone of immune checkpoint research.
| Target | Programmed cell death 1 ligand 1 (gene CD274; UniProt Q9NZQ7, human) |
|---|---|
| Clone | IDC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.3 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human Ramos cells, human HepG2 cells, human THP-1 cells, human K562 cells, rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:100 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human thymoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PD-L1 (CD274).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places programmed cell death 1 ligand 1 in the cell membrane, early endosome membrane, recycling endosome membrane and nucleus. Tissue expression noted by UniProt (human): Highly expressed in the heart, skeletal muscle, placenta and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jihua Tian et al. (2024) Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma. Journal for ImmunoTherapy of Cancer. 10.1136/jitc-2023-008226
Jiaming He et al. (2020) Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2020.110541
Pu Ning et al. (2018) CD25 and TGF-β blockade based on predictive integrated immune ratio inhibits tumor growth in pancreatic cancer. Journal of Translational Medicine. 10.1186/s12967-018-1673-6
Huang Wei et al. (2023) IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer. BMC Cancer. 10.1186/s12885-022-10353-5
Yuxin Xie et al. (2026) Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis. Fitoterapia. 10.1016/j.fitote.2026.107085
Qingyuan Li et al. (2018) Expression of B7 Homolog 1 (B7H1) Is Associated with Clinicopathologic Features in Urothelial Bladder Cancer. Medical Science Monitor. 10.12659/MSM.910956
Wang YH et al. (2014) Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance. Asian Pacific Journal of Cancer Prevention. 10.7314/apjcp.2014.15.3.1321
