| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human PD-L1 (CD274) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CD274 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone IDC-3; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody catalog # M00109. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CD274 (Programmed cell death 1 ligand 1).
- Antibody format: Monoclonal; clone IDC-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CD274 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. . Reported cellular localization context: Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. .
Research relevance and current trends
- Research context keywords from the source record include: Autoimmune,Cancer,Cell Type Markers,Immune System Diseases,Immunology,Tumor Antigens,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate CD274 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CD274 expression by Western blot in cell or tissue lysates, Detect CD274 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 40 kDa; calculated MW: 33275 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 40 kDa
- Cellular localization (provided): Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Tissue details (provided): Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
