| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3',5'-cyclic-AMP phosphodiesterase 4B; DPDE4; PDE32; cAMP-specific phosphodiesterase 4B; PDE4B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDE4B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25P94 is a rabbit monoclonal antibody that recognizes 3',5'-cyclic-AMP phosphodiesterase 4B (gene PDE4B) in human and mouse samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 83.3 kDa, and the supplier reports an observed band at about 95 kDa.
PDE4B is a cAMP-specific phosphodiesterase of the PDE4 family that terminates cAMP signaling. It is prominent in immune cells and the brain, where it helps control inflammatory cytokine production and neuronal signaling, and it is one of the targets through which PDE4 inhibitors exert anti-inflammatory and central nervous system effects.
| Target | 3',5'-cyclic-AMP phosphodiesterase 4B (gene PDE4B; UniProt Q07343, human) |
|---|---|
| Clone | 25P94 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.3 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PDE4B.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Expressed in brain, heart, lung and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
