| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3-phosphoinositide-dependent protein kinase 1;hPDK1;2.7.11.1;PDPK1;PDK1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PDPK1, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 3-phosphoinositide-dependent protein kinase 1 (gene PDPK1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 63.2 kDa, and the supplier reports an observed band at about 70 kDa.
PDPK1 (3-phosphoinositide-dependent protein kinase 1, often called PDK1) is a master kinase of the AGC family. It phosphorylates the activation loop of AKT, S6K, RSK, SGK, PKC isoforms and other kinases, so it is central to insulin and growth factor signaling through PI3K, controlling cell growth, survival and metabolism, and is widely studied in cancer and metabolic research.
| Target | 3-phosphoinositide-dependent protein kinase 1 (gene PDPK1; UniProt O15530, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 63.2 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL |
| Western blot | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue, COLO320 cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat testis tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue, mouse small intestine tissue.
Immunogen
Synthetic peptide from the C-terminal region of human PDPK1. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 3-phosphoinositide-dependent protein kinase 1 in the cytoplasm, nucleus, cell membrane and cell junction. Tissue expression noted by UniProt (human): Appears to be expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Na Yang et al. (2025) Bioactive compound combinations from Rhodiola tangutica alleviate pulmonary vascular remodeling in high-altitude pulmonary hypertension rats through the PI3K–AKT pathway. Frontiers in Pharmacology. 10.3389/fphar.2025.1582677
