| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxisome proliferator-activated receptor gamma coactivator 1-beta; PGC-1-beta; PPAR-gamma coactivator 1-beta; PPARGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PPARGC1B; PERC; PGC1; PGC1B; PPARGC1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PGC1 beta/PPARGC1B recombinant protein (Position: A8-P375). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects peroxisome proliferator-activated receptor gamma coactivator 1-beta (gene PPARGC1B) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 113.2 kDa, and the supplier reports an observed band at about 113 kDa.
PGC-1β (PPARGC1B) is a transcriptional coactivator closely related to PGC-1α. It enhances the activity of estrogen receptor α, NRF1 and other factors and supports basal mitochondrial biogenesis and oxidative metabolism. It is studied in lipid metabolism, mitochondrial function and energy balance.
| Target | Peroxisome proliferator-activated receptor gamma coactivator 1-beta (gene PPARGC1B; UniProt Q86YN6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 113.2 kDa |
| Observed MW | 113 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, human HEK293 cells, human U2OS whole cellhuman MDA-MB-453 cells, human K562 cells, rat brain tissue, rat skeletal muscle tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U937 cells, HEPA1–6 cells.
Immunogen
Recombinant human PGC1 beta/PPARGC1B fragment (Ala8–Pro375), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peroxisome proliferator-activated receptor gamma coactivator 1-beta in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in heart, brain and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuemei Liao et al. (2010) Troglitazone induces cytotoxicity in part by promoting the degradation of peroxisome proliferator-activated receptor γ co-activator-1α protein. British Journal of Pharmacology. 10.1111/j.1476-5381.2010.00900.x
Tang Han et al. (2022) Arbutin Protects Retinal Pigment Epithelium Against Oxidative Stress by Modulating SIRT1/FOXO3a/PGC-1α/β Pathway. Frontiers in Genetics. 10.3389/fgene.2022.922807
Minghui Gao et al. (2011) Mitogen-activated protein kinase kinases promote mitochondrial biogenesis in part through inducing peroxisome proliferator-activated receptor γ coactivator-1β expression. Biochimica et Biophysica ACTA-Molecular Cell Research. 10.1016/j.bbamcr.2011.03.017
