| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Catenin beta-1;Beta-catenin;CTNNB1;CTNNB;OK/SW-cl.35, PRO2286; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-beta Catenin (S33/S37) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-beta Catenin (S33/S37) CTNNB1 Rabbit Monoclonal Antibody is an antibody targeting CTNNB1. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ICG-3; isotype: Rabbit IgG; reactivity: Human,Rat; observed MW: 53 kDa; calculated MW: 85497 MW.
Boster Bio Anti-Phospho-beta Catenin (S33/S37) CTNNB1 Rabbit Monoclonal Antibody catalog # P00004. Tested in WB application. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: CTNNB1 — Catenin beta-1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ICG-3; Isotype: Rabbit IgG
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 53 kDa; Calculated: 85497 MW
- Phospho site(s): S33,S37
Biological background
Protein function (datasheet): Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). .
Cellular localization (datasheet): Cytoplasm. Nucleus . Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. .
Tissue details (datasheet): Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .
Research relevance and current trends
- Commonly studied in contexts related to Actin, etc.,Atherosclerosis,Cadherins,Cancer,Cardiovascular,Cell Adhesion,Cytoplasmic,Cytoskeleton,Cytoskeleton/ECM,Invasion/Microenvironment,Microfilaments,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction,Signaling Pathways,Stem Cells,Vascular Inflammation.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
