| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3; HER3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ErbB3 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22E60 is a rabbit monoclonal antibody that recognizes receptor tyrosine-protein kinase erbB-3 (gene ERBB3) phosphorylated at Tyr1222 in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 148.1 kDa, and the supplier reports an observed band at about 185 kDa.
HER3 (ERBB3) is an EGFR-family receptor for neuregulins with weak intrinsic kinase activity. It signals mainly as a heterodimer with HER2, which phosphorylates HER3 and creates docking sites for PI3K, making HER3 a strong activator of AKT signaling. It is studied in development and in resistance to targeted cancer therapies.
| Target | Receptor tyrosine-protein kinase erbB-3 (gene ERBB3; UniProt P21860, human) — phosphorylated at Tyr1222 |
|---|---|
| Clone | 22E60 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 148.1 kDa |
| Observed MW | 185 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): SKBR3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human erbB3.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Epithelial tissues and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
