| Field | Specification |
|---|---|
| Target | |
| Alternative names | NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-IKB alpha (S36) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25N33 is a rabbit monoclonal antibody that recognizes NF-kappa-B inhibitor alpha (gene NFKBIA) phosphorylated at Ser36 in human and mouse samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 35.6 kDa, and the supplier reports an observed band at about 39 kDa.
IκBα (NFKBIA) holds NF-κB dimers such as p65/p50 in the cytoplasm by hiding the signals they need for nuclear import. When inflammatory stimuli activate the IKK complex, IκBα is phosphorylated and degraded, releasing NF-κB into the nucleus, so its phosphorylation and loss are standard readouts of NF-κB activation.
| Target | NF-kappa-B inhibitor alpha (gene NFKBIA; UniProt P25963, human) — phosphorylated at Ser36 |
|---|---|
| Clone | 25N33 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.6 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human RT4 cells, human HepG2 cells, human SIHA cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser36 of human IKB alpha.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places NF-kappa-B inhibitor alpha in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rui Tang et al. (2026) Exosome membrane-biomimetic nanomedicine targets the pre-metastatic niche via NF-κB inhibition to suppress breast cancer lung metastasis. Materials Today Bio. 10.1016/j.mtbio.2026.102856
Junhan Cao et al. (2025) Sea Cucumber Fucoidan Inhibits Helicobacter pylori Gastritis via MAPK/NF-κB Signaling and Gut Microbiota Modulation. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.5c02190
Chuang Liu et al. (2022) An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties. Foods. 10.3390/foods11162488
Jianing Tan et al. (2023) Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. International Journal of Molecular Medicine. 10.3892/ijmm.2023.5307
Yue Xu et al. (2021) Nootkatone protects cartilage against degeneration in mice by inhibiting NF-κB signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2021.108119
Shu Chi et al. (2022) Blueberry anthocyanin extracts protect against Helicobacter pylori-induced peptic epithelium injuries both in vitro and in vivo: the key role of MAPK/NF-κB pathway. European Journal of Nutrition. 10.1007/s00394-022-02830-1
Gaode Zou et al. (2021) Pioglitazone Ameliorates Renal Ischemia-Reperfusion Injury via Inhibition of NF-κB Activation and Inflammation in Rats. Frontiers in Physiology. 10.3389/fphys.2021.707344
Caihong Wang et al. (2021) Epigenetic regulation is involved in traffic-related PM2.5 aggravating allergic airway inflammation in rats. Clinical Immunology. 10.1016/j.clim.2021.108914
Jianwei Liu et al. (2018) Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF-κB pathway. Journal of Cellular Physiology. 10.1002/jcp.27650
Juan-juan Li et al. (2018) Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish. Fish & Shellfish Immunology. 10.1016/j.fsi.2018.09.032
