Anti-Phospho-IKB alpha (S36) Rabbit Monoclonal Antibody

Overview

This product is an anti-NFKBIA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25N33; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-Phospho-IKB alpha (S36) Rabbit Monoclonal Antibody catalog # P01139-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Key elements and design rationale

• Target: NFKBIA (NF-kappa-B inhibitor alpha).
• Antibody format: Monoclonal; clone 25N33; isotype IgG.
• Host: Rabbit.
• PTM context: Phospho site information provided: S36.
• Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

NFKBIA is a commonly studied target in molecular and cellular biology. Functional context (as provided): Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. . Reported cellular localization context: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. . Tissue expression notes (as provided): Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,TLR Signaling.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
• When phosphorylation is relevant, researchers frequently track stimulus-driven changes and compare phospho-state to total protein abundance.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate NFKBIA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NFKBIA expression by Western blot in cell or tissue lysates, Quantify NFKBIA-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 39 kDa; calculated MW: 35609 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 39 kDa
• Cellular localization (provided): Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .
• Tissue details (provided): Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.