| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like 1;FOXO3;FKHRL1, FOXO3A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-NAK/TBK1 (S172) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TBK1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23T28; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody catalog # P00261. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TBK1 (Forkhead box protein O3).
- Antibody format: Monoclonal; clone 23T28; isotype IgG.
- Host: Rabbit.
- PTM context: Phospho site information provided: S172.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TBK1 is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. . Reported cellular localization context: Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Tissue expression notes (as provided): Ubiquitous. .
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Associated Proteins,Cancer,Cell Biology,Co-Activators/Co-Repressors,Domain Families,Epigenetics and Nuclear Signaling,Forkhead Box,Hypoxia,Intracellular,Metabolism,Metabolism Processes,Nuclear Receptors,Nuclear Signaling Pathways,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Transcription,Transcription Factors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
- When phosphorylation is relevant, researchers frequently track stimulus-driven changes and compare phospho-state to total protein abundance.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TBK1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TBK1 expression by Western blot in cell or tissue lysates, Enrich TBK1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 84 kDa; calculated MW: 71277 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 84 kDa
- Cellular localization (provided): Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
- Tissue details (provided): Ubiquitous. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
