| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; TBK1; NAK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-NAK/TBK1 (S172) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23T28 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase TBK1 phosphorylated at Ser172 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 84 kDa.
TBK1 is a serine/threonine kinase that drives type I interferon responses to viral and bacterial infection. Recruited by adaptors such as STING, MAVS and TRIF, it phosphorylates IRF3 and IRF7 so they can enter the nucleus and induce interferon genes, and it also regulates selective autophagy; TBK1 variants are linked to ALS and frontotemporal dementia.
| Target | Serine/threonine-protein kinase TBK1 (gene TBK1; UniProt Q9UHD2, human) — phosphorylated at Ser172 |
|---|---|
| Clone | 23T28 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 84 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells, human U-87MG cells, human SH-SY5Y cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser172 of human NAK/TBK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase TBK1 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kai Huang et al. (2026) Cytoplasmic relocation of nuclear Ku70 by Bruceine A augments cGAS–STING pathway-mediated anti-tumor immunity. Acta Pharmaceutica Sinica B. 10.1016/j.apsb.2026.06.010
Zhenqi Zuo et al. (2024) Salvianolic acid A prevents UV-induced skin damage by inhibiting the cGAS-STING pathway. International Immunopharmacology. 10.1016/j.intimp.2024.111971
Hao Jinfang et al. (2024) TLR4 signalling: the key to controlling EV71 replication and inflammatory response. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2024.1393680
XI ZHU et al. (2025) Death domain-associated protein (Daxx) impairs colon cancer chemotherapy by inhibiting the cGAS-STING pathway. Oncology Research. 10.32604/or.2024.054930
Xiang Cai et al. (2024) JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway. Molecular Carcinogenesis. 10.1002/mc.23753
