| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1; PHE1A |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-PDHA1 (S293) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25P34 is a rabbit monoclonal antibody that recognizes pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (gene PDHA1) phosphorylated at Ser293 in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 43.3 kDa, and the supplier reports an observed band at about 43 kDa.
PDHA1 is the alpha subunit of E1, the first catalytic component of the mitochondrial pyruvate dehydrogenase complex. With PDHB it uses thiamine pyrophosphate to decarboxylate pyruvate, the step that commits glycolytic carbon to acetyl-CoA and the TCA cycle. Its activity is switched off by PDK phosphorylation, and PDHA1 deficiency is a well-known cause of lactic acidosis.
| Target | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (gene PDHA1; UniProt P08559, human) — phosphorylated at Ser293 |
|---|---|
| Clone | 25P34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.3 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human 293T cells, human A549 cells, human HeLa cells, human HepG2 cells, rat brain tissue, rat kidney tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser293 of human PDHA1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
