| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;3.1.3.16;Replication protein C;RP-C;PPP2CA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-PDHA1 (S293) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PDHA1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25P34; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody catalog # P01906. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PDHA1 (Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform).
- Antibody format: Monoclonal; clone 25P34; isotype IgG.
- Host: Rabbit.
- PTM context: Phospho site information provided: S293.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PDHA1 is a commonly studied target in molecular and cellular biology. Functional context (as provided): PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. . Reported cellular localization context: Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). . Tissue expression notes (as provided): Expressed in brain, and to a lesser extent in lung, kidney and testis.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cardiovascular,Cell Biology,Cell Cycle,Contractility,Cytoplasmic,Epigenetics and Nuclear Signaling,Intracellular Signaling,Kinases/Phosphatases,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Phosphatases,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
- When phosphorylation is relevant, researchers frequently track stimulus-driven changes and compare phospho-state to total protein abundance.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PDHA1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PDHA1 expression by Western blot in cell or tissue lysates, Detect PDHA1 in FFPE tissue sections by immunohistochemistry, Enrich PDHA1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 35594 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). .
- Tissue details (provided): Expressed in brain, and to a lesser extent in lung, kidney and testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
