| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipase D1;PLD 1;hPLD1;3.1.4.4;Choline phosphatase 1;Phosphatidylcholine-hydrolyzing phospholipase D1;PLD1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes phospholipase D1 (gene PLD1) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 124.2 kDa, and the supplier reports an observed band at about 124 kDa.
Phospholipase D1 hydrolyzes phosphatidylcholine to produce phosphatidic acid, a signaling lipid that influences membrane curvature and mTOR signaling. It is activated by ARF and Rho GTPases and PKC and takes part in vesicle trafficking, secretion and signal transduction, making it a target in cell signaling and cancer research.
| Target | Phospholipase D1 (gene PLD1; UniProt Q13393, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 124.2 kDa |
| Observed MW | 124 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): mouse cardiac muscle tissue, 22RV1 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human PLD1 fragment (Met1–His330), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.8% and 90.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places phospholipase D1 in the cytoplasm, endoplasmic reticulum membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qi Sun et al. (2024) Study on low concentration deltamethrin toxicity mediated by phospholipase D in Chinese mitten crab (Eriocheir sinensis) ovary. Aquatic Toxicology. 10.1016/j.aquatox.2024.107090
