| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform;PI3-kinase subunit alpha;PI3K-alpha;PI3Kalpha;PtdIns-3-kinase subunit alpha;2.7.1.153;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;PtdIns-3-kinase subunit p110-alpha;p110alpha;Phosphoinositide-3-kinase catalytic alpha polypeptide;Serine/threonine protein kinase PIK3CA;2.7.11.1;PIK3CA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PIK3CA recombinant protein (Position: H936-N1068). Human PIK3CA shares 98% amino acid (aa) sequence identity with mouse PIK3CA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone WH-3 is a rabbit polyclonal antibody that recognizes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (gene PIK3CA) in human samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 124.3 kDa, and the supplier reports an observed band at about 124 kDa.
PIK3CA encodes p110α, the catalytic subunit of class IA PI3K that converts PI(4,5)P2 into PIP3 downstream of growth factor and insulin receptors. PIP3 recruits AKT and PDK1 to drive growth, survival and metabolism, and activating PIK3CA mutations are among the most frequent in human cancers, making it a major oncology target.
| Target | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (gene PIK3CA; UniProt P42336, human) |
|---|---|
| Clone | WH-3 |
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 124.3 kDa |
| Observed MW | 124 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human MCF-7 cells, human K562 cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Raji cells.
Immunogen
Recombinant human PIK3CA fragment (His936–Asn1068), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Zhang Dapeng et al. (2016) The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats. Cellular Physiology and Biochemistry. 10.1159/000447822
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Ting Zhang et al. (2022) Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation. Journal of Integrative Medicine-JIM. 10.1016/j.joim.2022.07.004
Peng Fang et al. (2019) ZWINT is the next potential target for lung cancer therapy. Journal of Cancer Research and Clinical Oncology. 10.1007/s00432-018-2823-1
Jingjing Qiao et al. (2019) Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway. OncoTargets and Therapy. 10.2147/OTT.S213421
Peng YK et al. (2020) Circular RNA hsa_circ_0010882 promotes the progression of gastric cancer via regulation of the PI3K/Akt/mTOR signaling pathway. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_202002_20165
J. Xin et al. (2019) Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_201904_17709
Qing-Gao Wang et al. (2021) miR‑320a in serum exosomes promotes myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells. Experimental and Therapeutic Medicine. 10.3892/etm.2021.10305
