| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Piezo2 Antibody is an antibody targeting Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B (also reported as Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B).
- Immunogen/epitope region: Located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined)..
- Homology note: Mouse, rat - 12/13 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- KO-validated: yes (validation context may be assay-dependent).
- Cited use: IP, IHC, ICC, Whole Tissue Staining (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Conceptual control: KO/KD samples provide orthogonal support for target assignment when available.
- Provided control suggestions: Negative control: BLP-PC090.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC090; Negative control: BLP-PC090.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
