| Field | Specification |
|---|---|
| Target | |
| Alternative names | Melanocyte protein PMEL;ME20-M;ME20M;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;M-alpha;95 kDa melanocyte-specific secreted glycoprotein;P26;Secreted melanoma-associated ME20 antigen;ME20-S;ME20S;M-beta;PMEL;D12S53E, PMEL17, SILV; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PMEL17 / GP100 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AHH-16 is a rabbit monoclonal antibody that recognizes melanocyte protein PMEL in human samples and is validated for Western blot and IHC. The predicted molecular weight is 70.3 kDa, and the supplier reports an observed band at about 100 kDa.
PMEL (also called gp100 or Pmel17) is a melanocyte transmembrane protein whose processed fragments form functional amyloid fibrils inside melanosomes. These fibrils shape early melanosomes and provide a scaffold for melanin deposition, and gp100 is also a well-known melanoma antigen used in pathology and immunotherapy research.
| Target | Melanocyte protein PMEL (gene PMEL; UniProt P40967, human) |
|---|---|
| Clone | AHH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.3 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human melanoma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human melanoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PMEL17 / GP100.
Reactivity Notes
The supplier lists reactivity with human. UniProt places melanocyte protein PMEL in the endoplasmic reticulum membrane, endosome, melanosome and extracellular vesicle. Tissue expression noted by UniProt (human): Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
