Anti-PON1 Antibody (monoclonal, 11H2)

SKU:BHA21001762
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-Pon1 antibody (Mouse monoclonal, clone 11H2, Mouse IgG2a). Validated for ELISA. Reactivity: Mouse, Rat. Recognizes serum paraoxonase/arylesterase 1. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Pon1
Clone number 11H2
Host Mouse
Reactivity Mouse,Rat
Isotype Mouse IgG2a
Application(s) ELISA
Options selector
Catalog no. Size Conjugation
M00516-2 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: ELISA (Cap)
      Application details: ELISA (Cap), 1-5μg/mlContents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Pon1
Alternative names Serum paraoxonase/arylesterase 1; PON 1
UniProt # P52430
Host Mouse
Clone 11H2
Clonality
  • Monoclonal
Isotype
  • Mouse IgG2a
Reactivity
  • Mouse
  • Rat
Applications
  • ELISA
Immunogen E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).
Molecular weight 39.6 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted, extracellular space.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00516-2
Main SKU BHA21001762
Mouse Monoclonal

Product Overview

Clone 11H2 is a mouse monoclonal antibody that recognizes serum paraoxonase/arylesterase 1 (gene Pon1) in mouse and rat samples and is validated for ELISA. The predicted molecular weight is 39.6 kDa.

Paraoxonase 1 (PON1) is a calcium-dependent esterase made in the liver and carried in blood on HDL particles. It hydrolyzes lactones and toxic organophosphate metabolites such as paraoxon, and it helps protect LDL from oxidation. PON1 is widely studied in lipid metabolism, oxidative stress, cardiovascular research and pesticide sensitivity.

Target Serum paraoxonase/arylesterase 1 (gene Pon1; UniProt P52430, mouse)
Clone 11H2
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG2a
Reactivity Mouse, Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 39.6 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA (capture antibody) 1–5 µg/mL

Immunogen

Recombinant mouse PON1 fragment (Ala30–Asp274), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with mouse and rat. UniProt describes serum paraoxonase/arylesterase 1 as a secreted protein. Tissue expression noted by UniProt (mouse): Plasma, liver, kidney, heart, brain, small intestine and lung.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA (capture antibody) 1–5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse, Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant mouse PON1 fragment (Ala30–Asp274), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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