| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serum paraoxonase/arylesterase 1; PON 1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse PON1 recombinant protein (Position: A30-D274). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Pon1 (Integrin beta-2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PON1 Antibody Picoband® (monoclonal, 11H2) catalog # M00516-2. Tested in ELISA applications. This antibody reacts with Mouse, Rat.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG2a
- Clone number: Clone: 11H2
- Immunogen / epitope context: E. coli-derived mouse PON1 recombinant protein (Position: A30-D274). (reported region: A30-D274).
- Molecular weight context: reported MW: 40 kDa; calculated MW: nan
- Reactivity: Mouse,Rat
- Applications: ELISA
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
Integrin beta-2; paraoxonase 1. Isocitrate dehydrogenase [NADP], mitochondrialis anenzymethat in humans is encoded by theIDH2gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. Functional note: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification. Reported localization: Secreted, extracellular space. Expression/tissue context: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how Pon1 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how Pon1 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how Pon1 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
